mouse polyclonal anti ifit3 Search Results


91
Bioss anti ifit3 ab
Anti Ifit3 Ab, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech anti ifit3
Anti Ifit3, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Atlas Antibodies endogenous peroxidases
Endogenous Peroxidases, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp cxcl9 hs00171065 m1
Gene Exp Cxcl9 Hs00171065 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp cxcl10 hs00171042 m1
Gene Exp Cxcl10 Hs00171042 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Santa Cruz Biotechnology mouse anti ifit3
Mouse Anti Ifit3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Novus Biologicals mouse anti ifit3 pe
Mouse Anti Ifit3 Pe, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech anti ifit2
Anti Ifit2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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91
OriGene ifit3
Figure 3. ZAP, <t>IFIT3,</t> and IFIT1 are dominant contributors to the IFN-mediated suppression of VEEV.
Ifit3, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Abnova mouse monoclonal anti–human ifit3 (h00003437-b01
Figure 3. ZAP, <t>IFIT3,</t> and IFIT1 are dominant contributors to the IFN-mediated suppression of VEEV.
Mouse Monoclonal Anti–Human Ifit3 (H00003437 B01, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson anti-ifit3 antibodies
Figure 3. ZAP, <t>IFIT3,</t> and IFIT1 are dominant contributors to the IFN-mediated suppression of VEEV.
Anti Ifit3 Antibodies, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
ABclonal Biotechnology ifit3
Transcription level verified by RT-qPCR. Total RNAs from aMDCK and sMDCK cells were extracted and RT-qPCR was carried out to examine the expression of genes including DDX58 (RIG-I), MDA5, MAVS, STAT1, ISG15, OAS1, OAS3, and <t>IFIT3.</t> The experiments were repeated three times independently. The mRNA level of aMDCK cells was set as 1. Canine GAPDH was used as the reference gene. * p < 0.05, ** p < 0.01, *** p < 0.001 (Student’s t -test). ns indicates no significance.
Ifit3, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+polyclonal+anti+ifit3/IFIT3+Rabbit+pAb/pmc10974347-98-22-23
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Image Search Results


Figure 3. ZAP, IFIT3, and IFIT1 are dominant contributors to the IFN-mediated suppression of VEEV.

Journal: EMBO reports

Article Title: Interferon inhibits a model RNA virus via a limited set of inducible effector genes.

doi: 10.15252/embr.202356901

Figure Lengend Snippet: Figure 3. ZAP, IFIT3, and IFIT1 are dominant contributors to the IFN-mediated suppression of VEEV.

Article Snippet: Primary antibodies probing for IRF9 (Proteintech Cat# 14167-1-AP, RRID:AB_2296227), STAT1 (Proteintech Cat# 66545-1-Ig, RRID:AB_2881907), STAT2 (Proteintech Cat# 16674-1-AP, RRID:AB_10644445), IFIT1 (Proteintech Cat# 23247-1-AP, RRID:AB_2811269), IFIT3 (OriGene Cat# TA500726, RRID:AB_11127767), ZAP (Proteintech Cat# 66413-1-Ig, RRID: AB_2881785), GAPDH (Proteintech Cat# 60004-1-Ig, RRID: AB_2107436), BST2/Tetherin (Proteintech Cat# 13560-1-AP, RRID: AB_2067220), RSAD2/Viperin (Proteintech Cat# 28089-1-AP, RRID: AB_2881057), ISG20 (Proteintech Cat# 22097-1-AP, RRID: AB_2878994), and IFITM3 (Proteintech Cat# 11714-1-AP, RRID: AB_2295684) were used in this study.

Techniques:

Figure 4. ZAP, IFIT3, and IFIT1 in combination exhibit differential antiviral activity toward diverse RNA viruses.

Journal: EMBO reports

Article Title: Interferon inhibits a model RNA virus via a limited set of inducible effector genes.

doi: 10.15252/embr.202356901

Figure Lengend Snippet: Figure 4. ZAP, IFIT3, and IFIT1 in combination exhibit differential antiviral activity toward diverse RNA viruses.

Article Snippet: Primary antibodies probing for IRF9 (Proteintech Cat# 14167-1-AP, RRID:AB_2296227), STAT1 (Proteintech Cat# 66545-1-Ig, RRID:AB_2881907), STAT2 (Proteintech Cat# 16674-1-AP, RRID:AB_10644445), IFIT1 (Proteintech Cat# 23247-1-AP, RRID:AB_2811269), IFIT3 (OriGene Cat# TA500726, RRID:AB_11127767), ZAP (Proteintech Cat# 66413-1-Ig, RRID: AB_2881785), GAPDH (Proteintech Cat# 60004-1-Ig, RRID: AB_2107436), BST2/Tetherin (Proteintech Cat# 13560-1-AP, RRID: AB_2067220), RSAD2/Viperin (Proteintech Cat# 28089-1-AP, RRID: AB_2881057), ISG20 (Proteintech Cat# 22097-1-AP, RRID: AB_2878994), and IFITM3 (Proteintech Cat# 11714-1-AP, RRID: AB_2295684) were used in this study.

Techniques: Activity Assay

Figure 5. ZAP, IFIT3, and IFIT1, but not BST2, RSAD2, ISG20, or IFITM3 are required for IFN-mediated suppression of VEEV production and spread.

Journal: EMBO reports

Article Title: Interferon inhibits a model RNA virus via a limited set of inducible effector genes.

doi: 10.15252/embr.202356901

Figure Lengend Snippet: Figure 5. ZAP, IFIT3, and IFIT1, but not BST2, RSAD2, ISG20, or IFITM3 are required for IFN-mediated suppression of VEEV production and spread.

Article Snippet: Primary antibodies probing for IRF9 (Proteintech Cat# 14167-1-AP, RRID:AB_2296227), STAT1 (Proteintech Cat# 66545-1-Ig, RRID:AB_2881907), STAT2 (Proteintech Cat# 16674-1-AP, RRID:AB_10644445), IFIT1 (Proteintech Cat# 23247-1-AP, RRID:AB_2811269), IFIT3 (OriGene Cat# TA500726, RRID:AB_11127767), ZAP (Proteintech Cat# 66413-1-Ig, RRID: AB_2881785), GAPDH (Proteintech Cat# 60004-1-Ig, RRID: AB_2107436), BST2/Tetherin (Proteintech Cat# 13560-1-AP, RRID: AB_2067220), RSAD2/Viperin (Proteintech Cat# 28089-1-AP, RRID: AB_2881057), ISG20 (Proteintech Cat# 22097-1-AP, RRID: AB_2878994), and IFITM3 (Proteintech Cat# 11714-1-AP, RRID: AB_2295684) were used in this study.

Techniques:

Transcription level verified by RT-qPCR. Total RNAs from aMDCK and sMDCK cells were extracted and RT-qPCR was carried out to examine the expression of genes including DDX58 (RIG-I), MDA5, MAVS, STAT1, ISG15, OAS1, OAS3, and IFIT3. The experiments were repeated three times independently. The mRNA level of aMDCK cells was set as 1. Canine GAPDH was used as the reference gene. * p < 0.05, ** p < 0.01, *** p < 0.001 (Student’s t -test). ns indicates no significance.

Journal: Vaccines

Article Title: Reduction in Interferon-Stimulated Genes Contributes to High-Yield Production of Influenza Virus in Suspension MDCK Cells

doi: 10.3390/vaccines12030287

Figure Lengend Snippet: Transcription level verified by RT-qPCR. Total RNAs from aMDCK and sMDCK cells were extracted and RT-qPCR was carried out to examine the expression of genes including DDX58 (RIG-I), MDA5, MAVS, STAT1, ISG15, OAS1, OAS3, and IFIT3. The experiments were repeated three times independently. The mRNA level of aMDCK cells was set as 1. Canine GAPDH was used as the reference gene. * p < 0.05, ** p < 0.01, *** p < 0.001 (Student’s t -test). ns indicates no significance.

Article Snippet: The following primary antibodies were used for detection: GAPDH (Transgene, Beijing, China), NS1 (Santa Cruz, CA, USA), RIG-I (Santa Cruz, CA, USA), IFIT3 (ABclonal, Wuhan, China), MAVS (Santa Cruz, CA, USA), and Flag (GenScript, Nanjing, China).

Techniques: Quantitative RT-PCR, Expressing

ISGs were decreased in sMDCK cells after viral infection. aMDCK and sMDCK cells were infected with the PR8 virus at an MOI of 0.01 for 48 h. Transcription levels of DDX58 (RIG-I), MDA5, MAVS, IFN-β, IFIT3, and ISG15 in aMDCK cells ( A ) and sMDCK cells ( B ) were detected by RT-qPCR. ( C ) Relative transcription levels of DDX58, MDA5, MAVS, IFN-β, IFIT3, and ISG15 in aMDCK cells and sMDCK cells. Canine GAPDH was used as the reference gene. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (Student’s t -test). ns indicates no significance. ( D ) Western blot was performed to detect RIG-I, IFIT3, MAVS, and GAPDH expressions in aMDCK and sMDCK cells after viral infection.

Journal: Vaccines

Article Title: Reduction in Interferon-Stimulated Genes Contributes to High-Yield Production of Influenza Virus in Suspension MDCK Cells

doi: 10.3390/vaccines12030287

Figure Lengend Snippet: ISGs were decreased in sMDCK cells after viral infection. aMDCK and sMDCK cells were infected with the PR8 virus at an MOI of 0.01 for 48 h. Transcription levels of DDX58 (RIG-I), MDA5, MAVS, IFN-β, IFIT3, and ISG15 in aMDCK cells ( A ) and sMDCK cells ( B ) were detected by RT-qPCR. ( C ) Relative transcription levels of DDX58, MDA5, MAVS, IFN-β, IFIT3, and ISG15 in aMDCK cells and sMDCK cells. Canine GAPDH was used as the reference gene. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (Student’s t -test). ns indicates no significance. ( D ) Western blot was performed to detect RIG-I, IFIT3, MAVS, and GAPDH expressions in aMDCK and sMDCK cells after viral infection.

Article Snippet: The following primary antibodies were used for detection: GAPDH (Transgene, Beijing, China), NS1 (Santa Cruz, CA, USA), RIG-I (Santa Cruz, CA, USA), IFIT3 (ABclonal, Wuhan, China), MAVS (Santa Cruz, CA, USA), and Flag (GenScript, Nanjing, China).

Techniques: Infection, Virus, Quantitative RT-PCR, Western Blot

RIG-I and IFIT3 inhibit viral infection in MDCK cells. ( A ) aMDCK cells were transfected with siRIG-I, siIFIT3, or siNC for 36 h. Cells were harvested and transcription levels of RIG-I and IFIT3 were detected by RT-qPCR. Canine GAPDH was used as the reference gene. ** p < 0.01, *** p < 0.001 (Student’s t -test). ns indicates no significance. ( B ) Western blot detection of RIG-I and IFIT3. ( C ) aMDCK cells were transfected with siRIG-I, siIFIT3, or siNC for 36 h and then infected with PR8 virus at an MOI of 0.001. Viral titers were determined by a TCID 50 assay at 24, 48, and 72 hpi. * p < 0.05, ** p < 0.01, *** p < 0.001 (Student’s t -test). ( D ) sMDCK cells were transfected with pCMV-3Flag-RIG-I, pCMV-3Flag-IFIT3, and pCMV-3Flag-EGFP for 36 h. Western blot was performed to detect RIG-I, IFIT3, and EGFP expressions. ( E ) sMDCK cells were transfected with pCMV-3Flag-RIG-I, pCMV-3Flag-IFIT3, and pCMV-3Flag-EGFP for 36 h and then infected with the PR8 virus at an MOI of 0.001. Viral titers were determined by a TCID 50 assay at 24, 48, and 72 hpi. * p < 0.05, ** p < 0.01, *** p < 0.001 (Student’s t -test).

Journal: Vaccines

Article Title: Reduction in Interferon-Stimulated Genes Contributes to High-Yield Production of Influenza Virus in Suspension MDCK Cells

doi: 10.3390/vaccines12030287

Figure Lengend Snippet: RIG-I and IFIT3 inhibit viral infection in MDCK cells. ( A ) aMDCK cells were transfected with siRIG-I, siIFIT3, or siNC for 36 h. Cells were harvested and transcription levels of RIG-I and IFIT3 were detected by RT-qPCR. Canine GAPDH was used as the reference gene. ** p < 0.01, *** p < 0.001 (Student’s t -test). ns indicates no significance. ( B ) Western blot detection of RIG-I and IFIT3. ( C ) aMDCK cells were transfected with siRIG-I, siIFIT3, or siNC for 36 h and then infected with PR8 virus at an MOI of 0.001. Viral titers were determined by a TCID 50 assay at 24, 48, and 72 hpi. * p < 0.05, ** p < 0.01, *** p < 0.001 (Student’s t -test). ( D ) sMDCK cells were transfected with pCMV-3Flag-RIG-I, pCMV-3Flag-IFIT3, and pCMV-3Flag-EGFP for 36 h. Western blot was performed to detect RIG-I, IFIT3, and EGFP expressions. ( E ) sMDCK cells were transfected with pCMV-3Flag-RIG-I, pCMV-3Flag-IFIT3, and pCMV-3Flag-EGFP for 36 h and then infected with the PR8 virus at an MOI of 0.001. Viral titers were determined by a TCID 50 assay at 24, 48, and 72 hpi. * p < 0.05, ** p < 0.01, *** p < 0.001 (Student’s t -test).

Article Snippet: The following primary antibodies were used for detection: GAPDH (Transgene, Beijing, China), NS1 (Santa Cruz, CA, USA), RIG-I (Santa Cruz, CA, USA), IFIT3 (ABclonal, Wuhan, China), MAVS (Santa Cruz, CA, USA), and Flag (GenScript, Nanjing, China).

Techniques: Infection, Transfection, Quantitative RT-PCR, Western Blot, Virus